Have you ever conducted an experiment that produced results that may not have turned out the way you wanted them to? Think about the things in your experimental environment that may have contributed to your "off" results. Would you ever suspect the lab equipment you use as the source of your error?
Well, scientists at the University of Alberta in Edmonton, Canada didn't either, until the experiment they were conducting about monoamine oxidase and how drugs affected this enzyme's activity produced some very odd results. Andrew Holt, the researcher who cast light on this issue, noticed that the enzyme was being inhibited at lower concentrations than it should have been, and began poking around to find out why this was happening.
Using common laboratory solvents such as water, DMSO and methanol, Holt cleaned out the plastic lab equipment he routinely used--things like plastic culture tubes and pipet tips. He discovered that there were several compounds that were washed out in the leachate (the resulting solution that was washed out of the equipment) that had the potential to affect the outcome of any experiment conducted with the equipment in question.
Because so much plastic labware is used in experimentation, Holt felt the need to record and share the data he'd collected with some of his colleagues to see if they'd experienced similar issues. Much to his surprise, they had.
Now, the implications for Holt's work are far-reaching. Think about all the labs conducting research in life science, biochemistry, genomics and other sciences that are likely to use plasticware as a part of their lab equipment. Think about why they use plasticware as opposed to glassware. How do you think that the presence of these organic leachates will change the way scientists do research? How will results already reported be treated by the scientific community, based on this new knowledge? How will this study affect the way scientists do their work?
Posted by scienceguru on November 7, 2008
Tags better living through biochem, discuss, science and society, what do you think?


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